Fig. 3.

Ectopic expression of mir-205 decreases tumor cell migration. A. ¹²⁵I-α2M (1 nM) uptake and degradation assays were performed at 37°C for 4 h in the absence or presence of RAP (500 nM). The degradation of ¹²⁵I-α2M was analyzed as described in the Materials and Methods section. B. Twenty-four hours after overexpression of control or miRNA, three-dimensional migration assays were performed using Boyden chambers. Representative photos of migration are shown taken at x200 magnification under an inverted microscope. The migrated cells were counted from five randomly chosen fields. Values are the average of triple determinations with the S.D. indicated by error bars. *p < 0.05