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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Aug 8;388(2):400–405. doi: 10.1016/j.bbrc.2009.08.020

Fig. 4.

Fig. 4

Deletion of MiR-205 seed site in LRP1 3′UTR restores target gene expression. A. Cells were transfected with mLRP4, mLRP4 containing LRP1 3′UTR, or mLRP4 containing LRP1 3′UTR with the miR-205 seed site deleted. Cells were harvested 48 h later and analyzed for expression levels by Western blot. B. Densitometric analysis of three independent Western blots. C. Luciferase assays were performed using cells expressing pMIR-Luc vector containing full-length or miR-205 seed site deleted sequences of LRP1 3′UTR. Cells were harvested 24 h later and analyzed for luciferase expression. β-gal expression vector was co-transfected for normalization of transfection efficiency. Values are the average of triple determinations with the S.D. indicated by error bars. *p < 0.05.