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NDPK immunoblots of ovine airway epithelial membrane protein separated on SDS-PAGE, showing consistent appearance of 51 kDa HMW bands in three independent preparations (lanes 1–3). Equivalent loadings of cytosolic (lane 4) and membrane (lane 5) protein showed that the HMW species is absent from the cytosolic fraction. The antibody used and the membrane preparation protocol are described in the methods for this and subsequent figures.
