Skip to main content
. 2009 Sep 22;4(9):e7111. doi: 10.1371/journal.pone.0007111

Figure 1. Characterization of the GEN3 plasmacytoid dendritic cell line.

Figure 1

The LPDC-derived cell line GEN3 or primary purified PDC were incubated for 18 hours in the presence of formaldehyde-inactivated influenza virus. GEN3 cells (A) or primary PDC (B) were stained with indicated mAb (open curves) or isotype control (filled curves) at day 0 (upper panels) and day 1 (lower panels). At day 1 production of IFNα by GEN3 was measured by ELISA (C) (mean±SD of four experiments), and cell morphology was examined after May-Gründwald Giemsa staining of cell cytospins (D). Data are representative of 3 independent experiments.