Table 1.
Construct | Specific activity (nM min−1 nM−1) |
---|---|
WT | 0.2289 ± 0.0242 |
ΔN | 0.0482 ± 0.024 |
ΔC | 0.084 ± 0.0318 |
WT + ssRNA | 0.1751 ± 0.0101 |
WT + dsRNA | 0.2227 ± 0.0421 |
WT + S5 | 0.4699 ± 0.0301 |
Specific activity is represented as the amount of Pi released (nM) for a given concentration of enzyme (nM) for a certain time (min). Experiments were conducted in duplicates and were reproduced at least twice. The errors represent the standard deviation from the average. Values were corrected for the background intrinsic GTP hydrolysis.