(A–C) CQ and NEM prevented the ACTH-induced increase in INa magnitude, whereas BFA did not prevent the ACTH-induced current enhancement. Potassium currents were blocked in all conditions with 60 mM TEA. I-V curves depict normalized INa at baseline and after 30 min exposure to CQ, NEM, or BFA, then 30 min after addition of ACTH. Insets: summary of peak INa at baseline and after 30 min exposure to CQ, NEM, or BFA, then 30 min after addition of ACTH. Asterisks indicate conditions significantly different from other conditions by Tukey's HSD following significant omnibus repeated measures ANOVA (for CQ F
[2,4,8] = 17.13, p<0.01; NEM F
[2,4,8] = 17.28, p<0.01; BFA F
[2,5,10] = 16.16, p<0.001).