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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Microbiol Res. 2008 Oct 23;164(1):1–8. doi: 10.1016/j.micres.2008.08.006

Figure 3. Interaction of YgfD and Sbm in vivo.

Figure 3

A. Proteins from MGC1876 crude cell lysate (lane 1) or immunoprecipitated from crude cell lysate with anti-YgfD (lanes 2–4) were analyzed by Coomassie blue staining (lanes 1 and 2) or Western blot analysis, probed with anti-Sbm (lane 3) or anti-YgfD (lane 4). Note anti-YgfD captures a complex of YgfD and Sbm (lane 2). B. Immunocapture of YgfD and Sbm using immobilized anti-Sbm (lanes 1–3) or anti-YgfD antibodies (lanes 4–6) followed by SDS-PAGE and Western blot analysis using anti-Sbm (upper) or anti-YgfD (lower). Lanes 1, 4, strain BL21; lanes 2, 5, MG1876; lanes 3, 6, MGC1876ΔygfD.