Fig. 2.

The resveratrol-induced apoptosis is mediated by activation of caspase-3. Confluent cells were incubated with 0.5% FBS-DMEM containing none (CON), 100 µM etoposide (ETO) or 100 µM resveratrol (RES) for 36 h. The cell were then stained with Hoechst 33258 and we observed the nuclei under a fluorescence microscope (A). Arrows indicate the fragmented nuclei. In panel B, after the cells were lysed, proteins in the cell lysates were resolved by SDS-PAGE, electrotransferred to PVDF membranes and immunoblotted with caspase-3 antibodies.