Fig. 4.

Resveratrol modulates a variety of cell signaling molecules. Serum-depleted cells (grown in 0.5% FBS-DMEM) were stimulated with 100 µM resveratrol treatment as a function of time. After the cells were lysed, the proteins in cell lysates were resolved by SDS-PAGE, electrotransferred to PVDF membranes and immunoblotted with the appropriate antibodies.