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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Acta Biomater. 2008 Dec 25;5(5):1389–1398. doi: 10.1016/j.actbio.2008.11.003

Table 1.

Characteristic HSA Adsorption Parameters by Radiometry and Depletion

Radiometry
Adsorbed Fraction Dmax (unit)
(N, Std. Dev.)
Γmax (unit)
(N, Std. Dev.)
S (unit)
(N, R2)*
(WB0)max(unit)
Bound - 4.20±0.59 (mg/m2) (18,0.92) 0.10±0.005 (mL/m2) (11,0.81) 42.39±6.32 (mg/mL)
Total 3.73±1.77(mg/mL) (17,0.70) - 0.08±0.005 (11,0.84) 46.16±22.07 (mg/mL)
6.54±2.60 (mg/m2) (17,0.70) - 0.14±0.009 (mL/m2) (13,0.84) 45.24±18.17 (mg/mL)
Electrophoresis
Total 15.97±1.73 (mg/mL) (18,0.89) - 0.41±0.022 (9,0.84) 38.74±4.67 (mg/mL)
28.46±3.08 (mg/m2) (18,0.89) - 0.73±0.039 (mL/m2) (9,0.84) 38.75±4.67 (mg/mL)

Notes: Bound = protein remaining after 3X PBS rinse. Total = protein depleted from solution.

*

(N, R2) = (number of data points, R2 of linear regression through the adsorption isotherm)