Table 1.
Radiometry | ||||
---|---|---|---|---|
Adsorbed Fraction |
Dmax (unit) (N, Std. Dev.) |
Γmax (unit) (N, Std. Dev.) |
S (unit) (N, R2)* |
|
Bound | - | 4.20±0.59 (mg/m2) (18,0.92) | 0.10±0.005 (mL/m2) (11,0.81) | 42.39±6.32 (mg/mL) |
Total | 3.73±1.77(mg/mL) (17,0.70) | - | 0.08±0.005 (11,0.84) | 46.16±22.07 (mg/mL) |
6.54±2.60 (mg/m2) (17,0.70) | - | 0.14±0.009 (mL/m2) (13,0.84) | 45.24±18.17 (mg/mL) | |
Electrophoresis | ||||
Total | 15.97±1.73 (mg/mL) (18,0.89) | - | 0.41±0.022 (9,0.84) | 38.74±4.67 (mg/mL) |
28.46±3.08 (mg/m2) (18,0.89) | - | 0.73±0.039 (mL/m2) (9,0.84) | 38.75±4.67 (mg/mL) |
Notes: Bound = protein remaining after 3X PBS rinse. Total = protein depleted from solution.
(N, R2) = (number of data points, R2 of linear regression through the adsorption isotherm)