Fig. 1. (A) EGF induces avβ5-mediated Rap1 activation and cell metastasis.
(A) EGF treatment increased activity of Rac1 and Rap1 in FG cells plated on anti-β5, but not anti-β1, integrin antibodies. (B) Rap1 knockdown blocked EGF-induced cell migration on vitronectin but not fibronectin. (C) Knockdown of β5 expression blocked EGF-induced pulmonary metastasis, but not primary tumor weight, in the chick CAM model. Mean±SEM, n≥6, *p≤0.05.