Table 1.
Carcinoma cells of distinct histological origin were analyzed for the role of Src in αvβ5-mediated migration. FACS analysis was used to determine αvβ5, β3 or β1 integrin expression on the cell surface. Requirement of Src kinase in cell migration was determined by stimulating cells with or without EGF (50ng/ml) and/or pretreated with Src inhibitor (SKI-606, 500nM). Cells were allowed to migrate toward vitronectin (VN) or fibronectin (FN).
| Carcinoma cell lines (orgin) |
FACS analysis of integrin beta subunits: |
Requirement of Src kinase for αvβ5- mediated migration (VN) |
Requirement of Src kinase for β1- mediated migration (FN) |
||
|---|---|---|---|---|---|
| β1 | β3 | β5 | |||
| FG (Pancreatic) | +++++ | - | ++ | +++ | - |
| Mia PaCa-2 (Pancreatic) | +++++ | -/+ | ++ | +++ | - |
| 2008 (Ovarian) | +++++ | - | ++ | +++ | - |
| MCF-7 (Breast) | +++++ | - | +++ | +++ | - |
| HT-29 (Colon) | +++++ | - | +++ | +++ | - |
| Panc-1 (Pancreatic) | +++++ | +++ | +++ | + | - |
| MDA-MB-435 (Breast) | +++++ | +++ | ++ | - | - |