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. 2008 Sep 17;28(38):9377–9385. doi: 10.1523/JNEUROSCI.3072-08a.2008

Figure 5.

Figure 5.

Baseline sleep phenotype produced by hyperpolarizing Tdc2-positive neurons. Female flies carrying a UAS–Kir2.1 transgene under the control of Tdc2–Gal4 flies were assayed for sleep. A, Four days of baseline sleep recording of 16 animals for each group. w;Tdc2–Gal4/Kir2.1;RC1 flies (gray dashed line) show significantly more sleep then their controls, w;RC1;RC1 (black solid line). Dark bars and white bars indicate nighttime and daytime, respectively. CT, Circadian time; ZT, Zeitgeber time. B, Total sleep is significantly increased in w;Tdc2–Gal4/Kir2.1;RC1flies (mean ± SEM; w;Tdc2–Gal4/Kir2.1;RC1, 829.19 ± 14.14, n = 47; W+;RC1;RC1, 655.35 ± 15.30, n = 47; p ≤ 0.0001, two-way ANOVA). The latency to sleep is significantly lower in Tdc2 × Kir2.1 flies (mean ± SEM; w;Tdc2–Gal4/Kir2.1;RC1, 13.86 ± 1.19, n = 47; w;RC1;RC1, 45.68 ± 8.40, n = 47; p ≤ 0.001, two-way ANOVA). C, Arousal threshold in w;Tdc2–Gal4/Kir2.1;RC1 flies. The animals were given three levels of stimulation to determine whether they were arousable. All animals that responded to the first stimulation were aroused on stronger stimulation. Compared with controls, an increased percentage of w;Tdc2–Gal4/Kir2.1;RC1 flies did not respond to any of the three levels of stimulation. At the strongest stimulation, more control flies responded than w;Tdc2–Gal4/Kir2.1;RC1 flies (w;Tdc2–Gal4/Kir2.1;RC1, n = 47; w;RC1;RC1, n = 47).