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. 2009 Sep 7;186(5):755–765. doi: 10.1083/jcb.200811058

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© 2009 Nguyen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — RGS2 amino acids 79–116 are sufficient to inhibit cellular protein synthesis. (A and B) H9c2 cardiomyoblasts (A) and MCF-7 adenocarcinoma cells (B) were infected with adenovirus (MOI = 4) encoding GFP (control), RGS2, or an RGS2 minigene for 48 h and used in a [³H]leucine protein synthesis assay as described in Materials and methods. The data were calculated as cpm/µg of total protein, normalized as a percentage of control, and are expressed as the mean ± SEM of five independent experiments performed in triplicate. *, P < 0.05 versus control (one-sample t test).