Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 7;186(5):685–692. doi: 10.1083/jcb.200906110

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Stagg et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — TRC8 forms a multimolecular ER complex with MHC I, US2, and SPP. (A) Doxycycline-induced, HA–ΔRING TRC8 HEK-293 cells transfected with US2 were radiolabeled for 30 min, and lysates were immunoprecipitated with HA or normal rabbit serum (NRS) control. (B) Doxycycline-induced HA–ΔRING TRC8 HEK-293 cells transfected with US2 and SPP were radiolabeled and immunoprecipitated with HA as in A, and samples were denatured and reprecipitated with normal rabbit serum, US2, SPP, or MHC I heavy chain (HCA2). (C) US2-expressing HeLa cells transfected with HA–ΔRING TRC8 were radiolabeled, and immunoprecipitations were performed as in B, or the cells were reprecipitated with MHC I heavy chain (HC10). (D) HA–ΔRING TRC8–overexpressing HEK-293 cells were radiolabeled, and immunoprecipitations were performed as in B. (A–D) Molecular mass is indicated in kilodaltons. 1°, primary; 2°, secondary; IP, immunoprecipitation.