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. 2009 Sep 7;186(5):739–753. doi: 10.1083/jcb.200902116

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© 2009 García-Cortés and McCollum

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Etd1 overexpression phenotype. (A) Cells of the indicated genotype were cultured at 25°C in the presence of thiamine and then cultured in the absence of thiamine for an additional 30 h to induce etd1⁺ expression. Samples were then fixed and visualized for GFP, DAPI, and CW fluorescence. (B) Strong overexpression of etd1⁺ does not change the levels of Spg1-GFP. spg1-GFP pREP3X (control [C]) or spg1-GFP pREP3X-etd1⁺ cells were grown as in A and harvested at the indicated times, and cell lysates were probed by Western blotting. (C) Cells of the indicated genotype were cultured and visualized as in A. w/o, without.