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. 2009 Sep 7;186(5):739–753. doi: 10.1083/jcb.200902116

Figure 6.

Figure 6.

SIN activity regulates both cortical and asymmetric localization of Etd1. (A) Reduced SIN activity causes prolonged localization of Etd1 to the cell cortex during anaphase/telophase. Cells of the indicated genotypes were grown at 25°C, stained, and visualized for GFP and CW fluorescence. (B) Cells with the indicated genotypes were grown as in A, and GFP-Etd1 images from a single medial focal plane are shown. Arrowheads indicate cells that display an asymmetrical localization of GFP-Etd1 in control cells, whereas asterisks mark symmetric localization in sid2-250 cells. (C) Interphase activation of the SIN causes loss of cortical and total GFP-Etd1. cdc16-116 nmt41-GFP-etd1 or cdc16-116 nmt41-GFP-etd1 cdc7-GFP cells were grown at 25°C and then shifted to 36°C for 2 h and visualized for GFP (right) and DIC images (left). GFP-Etd1 images from a single medial focal plane (top) and GFP-Etd1 and Cdc7-GFP z projection (bottom) are shown. Arrows indicate compartments that have a nucleus, as judged by nuclear exclusion of GFP-Etd1 or the presence of Cdc7-GFP. Arrowheads indicate compartments lacking a nucleus and SPB.