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. 2009 Sep 7;186(5):739–753. doi: 10.1083/jcb.200902116

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© 2009 García-Cortés and McCollum

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Asymmetric SIN signaling is required for Etd1 asymmetry. (A and B) Localization of Etd1 in S. pombe dikaryons with asymmetrically (A) or symmetrically localized Cdc7 (B) is shown. cdc11-123 cdc7-GFP nmt41-GFP-etd1 rlc1-mCherry cells were grown at 25°C and then shifted to 36°C for 2.5 h and finally to 25°C for an additional 2 h. Cdc7-GFP and GFP-Etd1 were then imaged by time-lapse microscopy at 25°C. Time is indicated in minutes.