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(Top) Schematic of stretching protocols, with λ, the global stretch ratio. Images were acquired from the shaded region. (Middle) Representative NLOM images of fibrillar collagen using SHG for biaxial stretches (λx, λy) of (1.01, 1.01) (A), (1.01, 1.1) (B), (1.01, 1.2) (C), (1.1, 1.2) (D), and (1.2, 1.2) (E). Scale bar represents 20 μm. (Bottom) Collagen fiber distribution and alignment indices (AIs). Error bars are standard errors (n = 5 images each).
