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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Eur J Pharmacol. 2009 Jun 17;616(1-3):31–37. doi: 10.1016/j.ejphar.2009.06.012

Fig. 2.

Fig. 2

NDGA has minimal effects on Sp1 mediated transcription but represses TGF-β activated transcription. A. 293 FT cells were transiently transfected with luciferase reporters pRL-null and Sp1-Luc or TATA-Luc. Cells were treated with different amounts of NDGA for 6 hours before being harvested for the dual luciferase assay. Luciferase activity of Sp1-Luc was normalized to TATA-Luc reporter activity. There was no effect on transcription by NDGA treatment at 10 and 30 µM, and 100 µM. B. Panc-1 cells were transiently transfected with luciferase reporters pRL-null and CAGA12-Luc or TATA-Luc. Cells were treated with 10 ng/ml TGF-β and different amounts of NDGA for 6 hours before being harvested for the dual luciferase assay. Luciferase activity of CAGA12-Luc was normalized to TATA-Luc reporter activity. TGF-β treatment induced activation of the reporter, and NDGA repressed this activation in a dose-dependent manner.