Mice (n=8) received baseline in vivo micro-CT scans before sham or PE surgeries to induce osteolysis over 10 days, and were given PBS, OPG, low dose ZA, high dose ZA or Aln treatment as indicated. Afterwards, the mice received an in vivo micro-CT scan to quantify volumetric osteolysis as described in Figure 2. Representative reconstructed images of the calvaria and osteolytic volume from each group are shown (A). The osteolytic volumes from each group are presented as the mean +/− SD (* p<0.05, ** p<0.01, *** p<0.001 vs. PBS control with Tukey test) (B).