Figure 3. Lentiviral vector-mediated GFP expression in cardiac cholinergic neurons.

Transgene expression was detected 10 days post-transduction with Lenti.EF1α-eGFP. A, GFP expression. B, same tissue sample stained with cholinergic neuron marker anti-CHAT. C, same tissue sample photographed under phase contract normal light. Note all CHAT-positive neurons (red cells in B) were co-localised with eGFP-expressing cells (green cells in A). Scale bar, 50 μm for all the images.