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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 16;386(3):477–482. doi: 10.1016/j.bbrc.2009.06.059

Fig. 4.

Fig. 4

X-gal staining of fracture callus harvested from Rosa26 LacZ; Prx1CreER-GFP mice. (A) Fracture callus of the ulna at 7 days after fracture. 1 mg/100μl tamoxifen was injected daily into the peritoneal cavity between P28–37. Fracture was created in the diaphysis of ulna at P33. Fracture callus was harvested at P40. Sections were counterstained with hematoxylin, eosin and alcian blue. (B–D) Higher magnification of the boxed areas in (A). X-gal stained cells were observed in undifferentiated cells at the fracture site (B), periosteal cells overlying the fracture callus (arrowheads in C, D), chondrocytes in the cartilaginous callus (arrows in C), and osteoblasts lining the bone trabeculae (arrows in D). (E) Fracture callus of the femur at 10 days after fracture. Femoral fracture was created at P49, and fracture callus was harvested at P59. 1 mg/100μl tamoxifen was injected into the peritoneal cavity at P52 and P53. Sections were counterstained with hematoxylin, eosin and alcian blue. (F) Higher magnification of the boxed area in (E). X-gal-stained cells were observed in the cartilaginous callus.