Table I.
Direct and competition ELISA of phages
| Phage | Normalized ELISA signal |
Electrostatic chargec |
|
|---|---|---|---|
| Directa | Competitionb | ||
| Strong β-Gal binders | |||
| DSLQASATd | 100 | 17 | −1.090 |
| ADLTVQAN | 97 | 2 | −1.090 |
| AELTTRAE | 94 | 3 | −1.086 |
| DQLNATAL | 113 | 1 | −1.090 |
| DQLTVSAQ | 84 | 18 | −1.090 |
| DSLHGQAM | 157 | 1 | −0.924 |
| DSLTLQAQ | 79 | 3 | −1.090 |
| DTLTHEAT | 63 | 2 | −1.921 |
| EDLTQRAL | 66 | 3 | −1.088 |
| SNLEMMAT | 100 | 1 | −1.088 |
| Clones which lost β-Gal binding ability | |||
| DDLTASAI | 1 | 130 | −2.090 |
| DELITEAH | <1 | 160 | −2.919 |
| DELTVAAN | 0 | 142 | −2.088 |
| DLLTTQAE | <1 | 94 | −2.088 |
| DNLEMMAQ | 1 | 104 | −2.088 |
| DNLITMAD | <1 | 105 | −2.090 |
| DSLNEQAV | 1 | 138 | −2.088 |
| DTLTENAV | 0 | 160 | −2.088 |
| EDLNAQAL | 1 | 113 | −2.088 |
| EDLSAMAG | 1 | 115 | −2.088 |
| EELESIAN | <1 | 129 | −3.083 |
| EELNGQAM | 1 | 130 | −2.086 |
| EELNQQAN | 1 | 104 | −2.086 |
| EELSNSAT | 2 | 123 | −2.086 |
| EELSQQAN | 1 | 153 | −2.086 |
| EELSVQAT | 1 | 107 | −2.086 |
| EELTLEAH | 1 | 146 | −2.917 |
| EELTNSAQ | <1 | 168 | −2.086 |
| ELLEAQAK | 2 | 94 | −1.087 |
| ELLTDQAS | <1 | 122 | −2.088 |
| ENLNEVAM | 1 | 130 | −2.086 |
| EQLENQAI | 2 | 123 | −2.086 |
| EQLEVQAS | 1 | 120 | −2.086 |
| EQLSSEAN | 1 | 179 | −2.086 |
| EQLSSNAE | 0 | 132 | −2.086 |
| EQLTVEAS | 2 | 113 | −2.086 |
| ESLAAEAT | 1 | 152 | −2.086 |
| ESLEAQAT | 1 | 137 | −2.086 |
| ESLTENAY | <1 | 133 | −2.087 |
| ETLENMAS | <1 | 97 | −2.086 |
| ETLEQQAT | 1 | 114 | −2.086 |
| ETLEVQAQ | 2 | 111 | −2.086 |
| ETLTESAA | 1 | 148 | −2.086 |
| LDLHEAAV | <1 | 135 | −1.921 |
| QELSIQAE | 1 | 119 | −2.086 |
| SLLSNQAE | 1 | 131 | −1.088 |
| Control phagee | <1 | 115 | −1.090 |
| No phage | 0 | 100 | – |
aELISA signals are normalized against the 1G40 phage ELISA signal. bELISA signals are normalized against the signal of the sample without phage. cThe summary electrostatic charges of 12–19 amino acids of pVIII were estimated by employing DNA Star software. dOriginal 1G40 phage with non-modified ‘wild-type’ domain C 12–19. eControl phage without β-Gal-binding peptide.