Fig. 1.

Analysis of DAV virions and structural proteins. (a) Sucrose gradient-purified virions were negatively stained and visualized by TEM at ×100 000 magnification. Bar, 100 nm. (b) Proteins present in purified virus particles were separated by electrophoresis through a 4–12 % Bis–Tris gradient gel. Total protein was visualized by staining with Coomassie blue. The capsid protein is indicated by an asterisk. Sizes of molecular mass markers are shown on the left. (c) Virion proteins separated through an SDS–10 % polyacrylamide gel were transferred to a nitrocellulose membrane and detected with a rabbit polyclonal anti-DAV antibody. The band marked with an asterisk corresponds to the 42 kDa protein indicated in (b).