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Analysis of DAV genomic nucleic acid. RNA was extracted from purified DAVHD virions and mock-treated (−) or treated with RNase ONE (Promega) (+), which degrades ssRNA only. As controls, a synthetic dsRNA product from DCV and the ssRNA genome from FHV were treated similarly. Following nuclease treatment, products were separated on a 1 % agarose gel and visualized by ethidium bromide staining. Sizes of the ssRNA markers (M; in nt) are indicated on the left.
