Fig. 5.

Organization of the DAV genome. (a) The 4806 nt DAV genome encodes two major ORFs and three additional small ORFs that contain >50 aa. The ORFs as shown start with an AUG codon; the extension (dotted line) on the capsid ORF indicates that this ORF overlaps the RdRP ORF, but the first methionine is downstream of the RdRP ORF. (b) Analysis of the 5′-proximal ORF showed high similarity of DAV RdRP regions to those of members of the families Tetraviridae and Birnaviridae. These viruses have a permuted RdRP, with the GDD region upstream of other palm subdomains. DAV RdRP also showed this permuted arrangement, as indicated by C–A–B. (c) MS analysis indicates that the 3′-proximal large ORF encodes the capsid protein. The deduced sequence of the capsid protein is shown and the peptides identified by MALDI-TOF (in bold type) and LC-MS/MS (underlined) are indicated.