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Validation of AP-4 binding partners from cICAT and iTRAQ analyses by Western blotting. Proteins enriched from HCT116 p53+/+ nuclear extracts by DNA pull-down (compare WT and MU) were analyzed by Western blotting using antibodies against selected proteins indicated at the left. The relative ratios of protein binding to WT and MU DNA-bead quantified by cICAT and iTRAQ analyses are shown at right. N/A, not available.
