Fig. 5.

Comparison of AP-4 and AP-4 truncation mutant binding partners by Western blotting. HCT116 p53^+/+ cells were transfected with empty vector, full-length (F.L.) AP-4 expression vector, or the indicated AP-4 truncation mutant expression constructs (top). After 48 h, nuclear extracts were prepared and subjected to DNA pull-down assays using WT DNA beads followed by Western blotting analysis using antibodies against selected proteins indicated at left. As a control, nuclear extracts were subjected to Western blotting using anti-actin antibody.