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Effect of TSA on AP-4-mediated repression of HDM2-P2 transcription. HCT116 p53+/+ and p53−/− cells were cotransfected with the wild-type HDM2-P2 reporter (hdm2luc01-WT) and the pcDNA-AP-4 expression construct. After 24 h, the indicated amounts of TSA were added to transfection reactions and incubated for additional 8 h or 24 h. Cells were harvested and subjected to (A) luciferase assays or (B) Western blotting using antibodies against acetyl-histone H3 or histone H3.
