Table II. Peptides of proteins identified in Table I modified by aHNE that are detected using peptide catch and photorelease.
All the observe precursor masses are within 10 ppm range of accuracy registered by a Thermo Orbitrap.
| IPI accession no. | Protein description | Adducted peptides | Peptide precursor calculated | Peptide precursor found | z | Residue number |
|---|---|---|---|---|---|---|
| IPI00021841 | ApoA1 | TH*LAPYSDELR | 806.9201 | 806.9237 | 2 | 162 |
| 538.2825 | 538.2843 | 3 | 162 | |||
| LEALK*ENGGAR | 490.2755 | 490.2772 | 2 | 182 | ||
| LAEYH*AK | 571.8139 | 571.8154 | 2 | 193 | ||
| ATEH*LSTLSEK | 763.9067 | 763.9084 | 2 | 199 | ||
| 509.6069 | 509.6078 | 3 | 199 | |||
| AK*PALEDLR | 441.9259 | 441.9278 | 3 | 208 | ||
| K*LNTQ | 457.7689 | 457.7712 | 2 | 239 | ||
| IPI00021854 | ApoA2 | VK*SPELQAEAK | 755.9274 | 755.9376 | 2 | 53 |
| SPELQAEAK*SYFEK | 646.6667 | 646.666 | 3 | 62 | ||
| SK*EQLTPLIK | 486.9642 | 486.964 | 3 | 69 | ||
| IPI00022229 | ApoB100 | VLVDH*FGYTK | 497.2732 | 497.2733 | 3 | 737 |
| DDKH*EQDMVNGIMLSVEK | 608.5425 | 608.5426 | 4 | 746 | ||
| LLDH*R | 482.7424 | 482.742 | 2 | 1214 | ||
| H*VGSK | 419.7427 | 419.7425 | 2 | 1229 | ||
| H*INIDQFVR | 484.9368 | 484.9363 | 3 | 2101 | ||
| VH*ELIER | 402.8995 | 402.8992 | 3 | 2342 | ||
| LKQH*IEAIDVR | 544.9859 | 544.983 | 3 | 2288 | ||
| SFDRH*FEK | 459.5701 | 459.5689 | 3 | 3207 | ||
| IPI00021885 | Fibrinogen α-chain precursor | TVIGPDGH*K | 617.8431 | 617.8444 | 2 | 475 |
| HRH*PDEAAFFDTASTGK | 733.359 | 733.3651 | 3 | 513 | ||
| TVTK*TVIGPDGHK | 555.3174 | 555.3192 | 3 | 467 | ||
| ESSSH*HPGIAEFPSR | 650.3219 | 650.326 | 3 | 563 | ||
| DSH*SLTTNIM̂EILR | 653.0068 | 653.0074 | 2 | 103 | ||
| VQH*IQLLQK | 473.289 | 473.2905 | 3 | 151 | ||
| SSSYSK*QFTSSTSYNR | 714.343 | 714.3467 | 3 | 581 | ||
| IPI00304273 | ApoA4 | K*LVPFATELHER | 584.3332 | 584.3328 | 3 | 79 |
| LNH*QLEGLTFQM̂K | 629.3329 | 629.3336 | 3 | 236 | ||
| LGPH*AGDVEGHLSFLEK | 706.3724 | 706.3724 | 3 | 332 | ||
| IPI00032258 | Complement C4-A precursor | SH*ALQLNNR | 455.2529 | 455.2526 | 2 | 1342 |
| DK*GQAGLQR | 428.5737 | 428.571 | 2 | 749 | ||
| IPI00553177 | Alpha-1-antitrypsin precursor | TDTSHHDQDH*PTFNK | 697.6557 | 697.6569 | 3 | 39 |
| TDTSH*HDQDHPTFNK | 697.6557 | 697.6566 | 3 | 34 | ||
| LYH*SEAFTVNFGDTEEAK | 790.3814 | 790.3777 | 3 | 163 | ||
| LVDK*FLEDVK | 506.2922 | 506.2941 | 3 | 153 | ||
| LGM̂FNIQHC*K | 506.5916 | 506.5936 | 3 | 255 | ||
| QINDYVEK*GTQGK | 597.6476 | 597.6481 | 3 | 187 | ||
| K*QINDYVEK | 483.2646 | 483.2589 | 3 | 179 | ||
| LQHLENELTH*DIITK | 529.5361 | 529.5406 | 4 | 293 | ||
| LQH*LENELTHDIITK | 705.7197 | 705.7221 | 3 | 286 | ||
| K*LSSWVLLM̂K | 511.3021 | 511.3018 | 3 | 258 | ||
| IPI00745872 | Isoform 1 of serum albumin precursor | SLH*TLFGDK | 443.5785 | 443.5793 | 3 | 90 |
| RH*PYFYAPELLFFAK | 737.3981 | 737.4041 | 3 | 169 | ||
| H*PYFYAPELLFFAK | 685.3644 | 685.3738 | 3 | 169 | ||
| LK*C#ASLQK | 629.863 | 629.8877 | 2 | 222 | ||
| LK*C#ASLQK | 420.2444 | 420.2453 | 3 | 222 | ||
| LK-C#ASLQK | 414.2409 | 414.2426 | 3 | 222 | ||
| RH*PDYSVVLLLR | 593.6807 | 593.681 | 3 | 361 | ||
| H*PDYSVVLLLR | 541.647 | 541.6522 | 3 | 361 | ||
| K-QTALVELVK | 474.6291 | 474.6292 | 3 | 548 | ||
| NLGK*VGSK | 557.335 | 557.3357 | 2 | 455 | ||
| SEVAH*R | 505.2749 | 505.2757 | 2 | 32 | ||
| IPI00478003 | α-2-macroglobulin precursor | TEH*PFTVEEFVLPK | 662.0191 | 662.0232 | 3 | 217 |
| GH*FSISIPVK | 465.938 | 465.937 | 3 | 523 | ||
| IPI00022426 | AMBP protein precursor | H*HGPTITAK | 424.9068 | 424.9061 | 3 | 141 |
| IPI00164623 | Complement C3 | AAVYHH*FISDGVR | 594.981 | 594.9813 | 3 | 918 |
| AAVYH*FISDGVR | 594.981 | 594.9809 | 3 | 918 | ||
| IPI00022488 | Hemopexin | GH*GHR | 437.7357 | 437.736 | 2 | 238 |
| IPI00022463 | Serotransferrin | DGAGDVAFVKH*STIFENLANK | 636.8328 | 636.8326 | 4 | 226 |
| EFQLFSSPH*GK | 529.9473 | 529.9433 | 3 | 308 | ||
| IPI00555812 | Vitamin d-binding protein precursor | H*LSLLTTLSNR | 522.6385 | 522.6435 | 3 | 208 |