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Isolation of sialylated O-linked glycopeptides from a fetuin tryptic digest using IP-NPLC with N-glycans first removed with PNGase F. Total ion mass spectrum from IP-NPLC-MS of peptides in the glycopeptide fraction from a 13.8 pmol fetuin tryptic digest N-glycan deglycosylated with PNGase F. Sample was injected with 1% HCl + 50 mm NH4HCO3 as the ion-pairing reagent. Ammonium adducts of glycopeptides are not labeled. Inset, MS2 spectrum of the ion at m/z 1111.95 (2+).
