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. 2009 Jun 12;8(9):2170–2185. doi: 10.1074/mcp.M900088-MCP200

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 7. — Multiple stage tandem MS analyses of differentially expressed tryptic glycopeptides of the wt and the pglD mutant of C. jejuni. MS² spectrum of the glycopeptide ion at (a) m/z 1036.22 (3+) from the wt; (b) the equivalent ion at m/z 1022.20 (3+) from the pglD mutant; (c) MS³ spectrum of the same ion at m/z 1022.20 (3+) from the pglD mutant. The MS³ fragment ions were matched to TDQNITLVAPPEFQK of the C. jejuni periplasmic protein, Cj1670c by database searching using MASCOT^TM with an ion score of 57 (NCBI accession no. YP_002345038). Bac, bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose); ^deAcBac, monoacetylated bacillosamine at the C-2 position only (2-acetamido-4-amino-2,4,6-trideoxyglucopyranose) (30, 39, 40).