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. 2009 Jun 12;8(9):2170–2185. doi: 10.1074/mcp.M900088-MCP200

Fig. 8.

Fig. 8.

Recovery and selectivity of IP-NPLC for isolating tryptic glycopeptides from complex peptide mixtures of the C. jejuni pglD mutant. Extracted ion chromatograms (XIC) of nanoRPLC-MS at m/z 1281.40 (3+) of a glycopeptide from the putative periplasmic protein, Cj0114, in the (a) Glycopeptide fraction; (b) Non-glycopeptide fraction; (c) Total digest. XICs of nanoRPLC-MS at m/z 1021.20 (3+) of a glycopeptide from protein export membrane protein, Cj0235c, in the (d) Glycopeptide fraction; (e) Non-glycopeptide fraction; (f) Total digest. XICs of nanoRPLC-MS at m/z 926.14 (3+) of a glycopeptide from the putative periplasmic protein, Cj0168c, in the (g) Glycopeptide fraction; (h) Non-glycopeptide fraction and (i) Total digest. The amino acid sequences of these glycopeptides are provided in Table III. The insets in (d), (e), and (f) show the combined mass spectra at m/z 1020.20 (3+) corresponding to the glycopeptide fraction, non-glycopeptide fraction, and total digest (23–24 min) for comparison. PA, peak area of the extracted ions; Nd, not detected; Fr., fraction.