Table IV. Quantitative analysis of glycoproteins from the periplasmic protein extracts of C. jejuni 11168 (wt) and the isogenic pglD mutant.
wt, wild-type; Intensities, peak area; S. D., standard deviation is based on triplicate nanoRPLC-MS analyses of triplicate IP-NPLC glycopeptide fractions; nd, not detected; —, a ratio is not given because the peptides were not detected in wt or mutant; ″, the same as above.
| Gene name | Protein name | Strain | Count | Intensities in wt | % error | Intensities in mutant | % error | Ratio wt/mutant | % error |
|---|---|---|---|---|---|---|---|---|---|
| (mean ± S. D.) | (mean ± S. D.) | (mean ± S. D.) | |||||||
| Cj0168c | (YP_002343627) putative periplasmic protein | wt | 1 | 16166 ± 503 | 3 | 1310 ± 157 | 12.0 | 12.34 ± 1.53 | 12.39 |
| ″ | ″ | pglD | 1 | 92 ± 18 | 19 | 1496 ± 250 | 16.7 | 0.06 ± 0.02 | 25.60 |
| Cj0235c | (NP_281430) protein export membrane protein | wt | 1 | 472 ± 26 | 6 | 27 ± 3 | 11.1 | 17.49 ± 2.17 | 12.41 |
| ″ | ″ | pglD | 1 | 70 ± 5 | 7 | 757 ± 126 | 16.6 | 0.09 ± 0.02 | 17.86 |
| Cj0200c | (YP_002343658) putative periplasmic protein | wt | 1 | 204 ± 87 | 43 | 416 ± 40 | 11.4 | 5.02 ± 2.22 | 44.33 |
| ″ | ″ | pglD | 1 | 204 ± 12 | 6 | 2033 ± 133 | 6.5 | 0.10 ± 0.01 | 8.78 |
| Cj0152c | (CAL34323) hypothetical protein | wt | 1 | 424 ± 103 | 24 | nd | only in wt | — | |
| ″ | ″ | pglD | 1 | nd | 242 ± 81 | 33.3 | only in mutant | — | |
| Cj1670c | (YP_002345038) putative membrane protein or CgpAc | wt | 1 | 224 ± 65 | 29 | 43 ± 4 | 10.1 | 5.22 ± 1.59 | 30.51 |
| ″ | ″ | pglD | 1 | 70 ± 5 | 7 | 1150 ± 114 | 9.9 | 0.06 ± 0.01 | 11.95 |
| Cj0864 | (YP_002343574) putative periplasmic protein | wt | 1 | 2186 ± 914 | 42 | 186 ± 9 | 5.1 | 11.71 ± 4.93 | 42.09 |
| ″ | ″ | pglD | 1 | nd | nd | not in both | — | ||
| Cj0114 | (YP_002344271) putative periplasmic protein | wt | 1 | 41 ± 5 | 11 | nd | only in wt | — | |
| ″ | ″ | pglD | 1 | 121 ± 20 | 16 | 1393 ± 137 | 9.8 | 0.09 ± 0.02 | 18.91 |
| Cj0114 | (YP_002344271) putative periplasmic protein | wt | 1 | 408 ± 167 | 41 | 32 ± 4 | 11.3 | 12.7 ± 5.42 | 42.42 |
| ″ | ″ | pglD | 1 | 115 ± 29 | 25 | 84 ± 16 | 19.3 | 1.37 ± 0.43 | 31.37 |
| Cj0783 | (YP_002344190) Periplasmic nitrate reductase cytochrome c-type subunit | wt | 1 | 527 ± 9 | 16 | nd | only in wt | — | |
| ″ | pglD | 1 | nd | nd | nd in both | — |