Fig. 2.

Validation of candidate T. brucei Nups. A, one copy of open reading frame Tb11.03.0140 (TbNup158) was genomically tagged at the COOH terminus with GFP. A montage of 21 confocal planes from the analysis of a TbNup158-tagged trypanosome in late anaphase is shown; each z-slice is 150 nm thick. There are ∼150 puncta associated with the nuclear envelope in this example. B, fluorescent microscopy gallery of COOH-terminal genomically labeled TbNups and corresponding 4′,6-diamino-2-phenylindole dihydrochloride (DAPI) fluorescence to visualize the DNA. Apart from TbSec13, which was labeled using the 3×HA epitope and visualized with a mouse monoclonal anti-hemagglutinin antibody at 1:1000, all other open reading frames were tagged with GFP. Scale bars, 2 μm.