Fig. 3.

TbNup92 exhibits cell cycle-dependent localization. A, a rabbit polyclonal antibody against HsNup107 (48) was used to stain a trypanosome cell bearing tagged TbNup89. Co-localization of these signals further supports assignment of the puncta as the trypanosome NPC (top). Two coiled coil TbNups, TbNup110 and TbNup92, only partially co-localize with this antibody and are found immediately to the nuclear side of the NPCs and adjacent to them, suggesting association with the nuclear basket of the NPC and consistent with potential similarity to Tpr (bottom). B, TbNup110-GFP and TbNup92-GFP, visualized in mitotic cells, demonstrate that although TbNup110 remains associated with the NPC throughout mitosis TbNup92 relocates to opposite poles in a region similar to the spindle attachment site. Scale bar, 2 μm. DAPI, 4′,6-diamino-2-phenylindole dihydrochloride.