Table I. Proteins identified from extracellular culture supernatant of intact P. falciparum-infected red blood cells by 2DE/MS and LC-MS/MS.

Mascot analysis used a probability-based molecular weight search (Mowse) score >30 to assign significance (p < 0.05) to the peptides identified. The gene locus as annotated in the Plasmodium database is indicated for each identified protein (PlasmoDB ID). The protein description is based on BLASTP search, database annotation, or protein sequence analysis in Pfam and NCBI conserved domain database. The predicted molecular mass (MM), isoelectric point (pI), signal peptide (SP), PEXEL motif, and transmembrane domain (TMD) of the proteins along with the parasite stage (R, ring; T, trophozoite; S, schizont) with maximum transcript level are indicated. For each protein, unique peptides identified (periods indicate cleavage sites), percent sequence coverage, number of SNPs, number of non-synonymous SNPs (nsSNPs) (causing change in amino acid sequence), Watterson';s θ (giving the genome-wide average population mutation rate), and orthologs in rodent malarial genomes have been indicated. Regions with consecutive polymorphic genes are highlighted in either light blue (with known antigen genes) or yellow (with unknown antigen genes). In blank cells are genes that were not sequenced or for which no good sequences were obtained. MFS, major facilitator superfamily.