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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6435–6446. doi: 10.4049/jimmunol.181.9.6435

Figure 5.

Figure 5

Responses to Lp5 in HCV naïve individuals derive from the naïve T cell population. A. Immunophenotyping of CD45RA+CCR7+ T-cells sorted cells showed a high purity of CD45RA+CCR7+CD28+CD62L+ naïve T cells (left panel), whereas a CD45RA-CCR7- control population was a CD45RO+ memory subpopulation (right panel). B. CD45RA+CCR7+ and CD45RA−CCR7− sorted T cells were expanded and restimulated for 2 cycles. Only the naïve CD45RA+CCR7+ population yielded a response to the Lp5 HCV epitope, as measured by magnitude (top) or percent positive (bottom). C. CD8+ cells were isolated by negative selection and then positively selected for CD62L or CD45RO expression. No Lp5-specific response was obtained from the CD45RO+ memory population, whereas a statistically significant number of wells were positive in the CD62L+ naïve group. D. Cells isolated by CD45RO depletion followed by CD8 positive selection were compared with CD8 cells obtained from PBMC by negative selection, depleted of CD45RO+ cells, then positively selected for CD62L expression. Following expansion and restimulation for 2 cycles, similar numbers of tetramer+ wells were detected from both populations containing naïve T cells.