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RANKL, OPG, and RANKL/OPG mRNA levels in ST-2 murine stromal cells after 3600 cycles of OFF. Loading frequencies of 1, 10, or 20 Hz were used. This resulted in loading durations of 60 min (1 Hz), 6 min (10 Hz), and 3 min (20 Hz). N = 4 for each group.
