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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Neurosci Methods. 2009 Mar 28;180(2):243–254. doi: 10.1016/j.jneumeth.2009.03.016

Figure 4.

Figure 4

Interstitial perfusion enhances viability of the culture. (a) Perfusion at 20μl/hr for 48 hours increased the viability of the cultures by 31.7%, compared to unperfused sister cultures, and to 30.7% compared to membrane insert controls. (p<0.01). A generalized linear model ANOVA followed by a Tukey’s multiple comparison test was used to evaluate statistical significance of the data. (b) Representative z-projection of unperfused culture (c) Representative z-projection of perfused culture. The cell death (red) is widespread in the unperfused cultures compared to perfused cultures. Images are ~440 μm across.