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Western blot analysis of the wild-type and mutant CLRN1-HA polypeptides. BHK-21 cells were transfected with the indicated HA-tagged CLRN1 plasmids. Nontransfected cells (0-BHK) were used as controls. Polypeptides were resolved on 12% SDS–PAGE, and anti-HA antibodies were used to probe the blots. Samples were untreated (-) or treated (+) with deglycosylating enzyme (PNGase F). The molecular weights of the protein bands are indicated on the left and right sides of the figure.
