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Three different methods for calculating relative protein content. (A) A stained gel showing partial separation of the α and β-MHC isoforms in samples of rat myocardium. (B) Enlarged and vertically scaled view of the demarcated region in panel A. ( C, D and E) Relative β-MHC content calculated using the Split method and the Gaussian and Lorentzian curve fits, respectively. A.U. stands for arbitrary units.
