Figure 2.

IL-4 induces the recruitment of neutrophil and monocytic cells in vivo. Murine air pouches were created and buffer, IL-4 (250 ng/ml) or LPS (1 μg/ml) was administered for 9 hours as described in Materials and Methods. (a) cell populations were identified by flow cytometry (n ≥ 6) after staining with purified rat antimouse 7/4 mAb directed against murine neutrophils (neutro, open curve) or rat antimouse F4/80 Ag Ab recognizing murine monocyte/macrophages (mono, open curve) as described in Materials and Methods. Inset, representative data plotted in the bar graph, where the grey curves illustrate the appropriate isotypic controls. (b) cell identification was confirmed by cytology. Results are from one representative experiment out of at least 6.