Figure 4.

The murine air pouch resident lining cells express the IL-4 receptor. Air pouches were created and lining cells were harvested as described in Materials and Methods. IL-4Rα chain was detected by RT-PCR (a) and by flow cytometry (b) using a specific antibody directed against murine IL-4Rα. Note that IL-4Rα mRNA was detected at variable levels in all tested animals, as well as IL-2Rγ. Only four different animals are presented (nos. 1–4). The receptor was detected on the cell surface of the lining cells. Results are from one representative experiment out of 4.