Table 2.
Analysis of the different analytes detected in the supernatant harvested from IL-4-induced lining cells1.
Analyte | 6 hours |
---|---|
BLC | 0.36 |
CD30L | 0.54 |
Eotaxin | 0.54 |
Eotaxin-2 | 2.92* |
Fas Ligand | 0.36 |
Fractalkine | 1.08* |
G-CSF | 1.20* |
GM-CSF | 0.72 |
IFNγ | 1.08* |
IL-1α | 0.98 |
IL-1β | 1.08* |
IL-2 | 1.08* |
IL-3 | 1.08* |
IL-6 | 0.85 |
IL-9 | 1.08* |
IL-10 | 1.08* |
IL-12 p40/p70 | 0.81 |
IL-12p70 | 0.81 |
IL-13 | 0.72 |
IL-17 | 0.54 |
I-TAC | 1.08* |
KC | 1.14* |
Leptin | 1.08* |
LIX/CXCL5 | 0.99 |
Lymphotactin | 0.54 |
MCP-1/CCL2 | 1.12* |
M-CSF | 0.54 |
MIG | 2.16* |
MIP-1α | 1.08* |
MIP-1γ | 1.03* |
RANTES | 0.52 |
SDF-1 | 2.16* |
TCA-3 | 0.75 |
TECK | 0.54 |
TIMP-1 | 0.65 |
TIMP-2 | 1.08* |
TNF-α | 0.54 |
sTNFR I | 1.24* |
sTNFR II | 1.08* |
1Air pouches were created and lining cells were harvested. pooled and incubated in vitro with 250 ng/ml IL-4 for 6 hours. The supernatant was used to probe the membranes (Mouse inflammation antibody array I) no more than two weeks after the in vivo experiments. Results are calculated as ratios, as described in Materials and Methods. *, ratio above 1; numbers in bold characters, ratio ≥ 1.2.