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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Biochem Pharmacol. 2009 Apr 24;78(4):344–354. doi: 10.1016/j.bcp.2009.04.016

Figure 2. Exposure to DCPIP dose-dependently induces apoptosis with cleavage of procaspase-3 and PARP in human A375 melanoma cells.

Figure 2

(A) Induction of cell death by exposure to increasing doses of DCPIP (20 and 40 µM, 24 h) in the absence or presence of the pancaspase inhibitor zVADfmk was assessed by flow cytometric analysis of annexinV-FITC/propidium iodide-stained cells. The numbers indicate viable cells (AV−, PI−, lower left quadrant) in percent of total gated cells (mean ± SD, n=3). Representative light microscopy pictures taken after 24 h exposure to DCPIP are shown in panels I-IV. (B) DCPIP-induced (10, 20, and 40 µM, 24 h) caspase-3 activation was examined by flow cytometric detection using an Alexa Fluor 488-conjugated monoclonal antibody against cleaved procaspase-3. One representative experiment of three similar repeats is shown. (C) DCPIP-induced (40 µM, 24 h) PARP cleavage was examined by immunoblot analysis.