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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Biochem Pharmacol. 2009 Apr 24;78(4):344–354. doi: 10.1016/j.bcp.2009.04.016

Figure 4. Genetic downregulation of NQO1 expression sensitizes human G361 melanoma cells to DCPIP-induced apoptosis.

Figure 4

(A) Induction of cell death by exposure to DCPIP (40 µM, 24 h) was examined in G361 wild type cells (untreated, wt) and after control siRNA treatment (siControl, siC) and NQO1 siRNA knockdown (siNQO1). The numbers indicate viable cells (AV−, PI−, lower left quadrant) in percent of total gated cells (mean ± SD, n=3). Representative light microscopy pictures taken after 24 h exposure to DCPIP are shown in panels I-III. (B) NQO1-Knockdown was confirmed by expression analysis using quantitative RT-PCR (mean ± SD, n=3) and (C) immunoblot analysis as specified in Materials and Methods.