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. Author manuscript; available in PMC: 2009 Sep 13.
Published in final edited form as: Am J Trop Med Hyg. 2006 Apr;74(4):578–584.

Figure 1.

Figure 1

Frequencies of dendritic cell subsets in whole blood. Peripheral blood mononuclear cells (PBMCs) were gated in R1 to exclude granulocytes. Dendritic cell (DC) markers such as CD1c and CD11c defining myeloid DCs (mDCs) and CD123 and BDCA2 defining plasmacytoid DCs (pDCs) can be expressed on subsets of T cells, monocytes, and B cells. It is therefore necessary to exclude these cell populations from analysis using a cocktail of antibodies against lineage markers for these subsets (CD3 for T cells, CD14 for monocytes, and CD19 for B cells). Within the R1 gate (gates are indicated by the rectangle and ovals), DCs are then defined as lineage marker negative, HLA DR-positive cells (R2). Within the R2 gate, the different DC subsets are defined as CD11c- and CD1c-positive mDC or CD123- and blood dendritic cell antigen 2 (BDCA2)–positive pDC. The absolute number of mDCs or pDCs is then calculated from their percentage in PBMCs using white blood cell counts. SSC = side scatter; FSC = forward scatter.