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. Author manuscript; available in PMC: 2009 Sep 14.
Published in final edited form as: Biochem Pharmacol. 2008 Mar 6;75(11):2204–2213. doi: 10.1016/j.bcp.2008.02.030

Fig. 7.

Fig. 7

Retinoids recruit RXRα and VDR to the promoter region of the CYP3A4 chromatin. HepG2 cells were treated with D3 (0.1 μM), retinoids (10 μM) or DMSO (0.1%) for 48 h. ChIP assays were performed as described under Materials and Methods. Anti-RXRα antibody (a, c) or anti-VDR antibody (e, g) was used to precipitate the DNA-protein complexes. DNA fragments containing pER6 or dXREM were PCR-amplified and analyzed on a 2% agarose gel. Normal IgG was used as nonimmune control (b, d, f, h); Total cell lysate (10%) was used as input (i, j).